MAK567
Pyruvate Dehydrogenase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
PDH Activity Assay Kit
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About This Item
UNSPSC Code:
41116104
NACRES:
NA.84
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
Quality Level
Gene Information
human ... PDHA1(5160), PDHA2(5161 ), PDHB(5162), PDHX(8050)
mouse ... PDHA1(18597), PDHA2(18598), PDHB(68263), PDHX(27402)
rat ... PDHA1(29554), PDHA2(117098), PDHB(289950), PDHX(311254)
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General description
Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD+ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO2. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.
Application
Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.
Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.
Features and Benefits
Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Other Notes
One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.
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Danger
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
监管及禁止进口产品
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Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
Kato M, et al.
Structure (Oxford, United Kingdom), 16(12), 1849-1859 (2008)
An improved spectrophotometric assay of pyruvate dehydrogenase in lactate dehydrogenase contaminated mitochondrial preparations from human skeletal muscle
Chretien D, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 240(2), 129-136 (1995)
A key role for mitochondrial gatekeeper pyruvate dehydrogenase in oncogene-induced senescence
Kaplon J, et al.
Nature, 498(7452), 109-112 (2013)
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
Domingo G J, et al.
European Journal of Biochemistry, 266(3), 1136-1146 (1999)
Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: mechanism for rate enhancement in a multimeric structure
Danson M J, et al.
Proceedings of the National Academy of Sciences of the USA, 75(11), 5386-5390 (1978)
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Instructions
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