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Merck
CN

MAK567

Sigma-Aldrich

Pyruvate Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

PDH Activity Assay Kit

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About This Item

UNSPSC Code:
41116104

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usage

sufficient for 100 colorimetric tests

Quality Level

100

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... PDHA1(5160), PDHA2(5161 ), PDHB(5162), PDHX(8050)
mouse ... PDHA1(18597), PDHA2(18598), PDHB(68263), PDHX(27402)
rat ... PDHA1(29554), PDHA2(117098), PDHB(289950), PDHX(311254)

General description

Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD+ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO2. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.

Application

Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.
Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.

Features and Benefits

Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Other Notes

One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H315,H319,H334,H341,H351

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000350102
0000350102

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