Hydroxyproline Assay Kit

Hydroxyproline, known chemically as 4-hydroxyproline, emerges through the enzymatic modification of proline after protein synthesis, a process termed post-translational hydroxylation. This modified amino acid is predominantly found within collagen, contributing significantly to its triple-helical structure and overall stability. The abundance of hydroxyproline confined almost exclusively to collagen renders it a reliable biomarker for assessing collagen abundance in biological tissues.

Elevations in hydroxyproline can be indicative of heightened collagen metabolism, often observed in conditions that prompt increased collagen synthesis or breakdown. This is particularly evident in scenarios of enhanced bone turnover, where hydroxyproline measurements in bodily fluids can serve as clinical biomarkers for bone resorption and overall bone health.

Collagen′s integral role in the extracellular matrix of connective tissues lends it the tensile strength essential for tissue integrity. In the cosmetic industry, the supplementation of collagen is believed to enhance skin firmness and combat signs of aging, due to the structural support provided by hydroxyproline-containing collagen fibers.

Suitable for measuring hydroxyproline in various biological samples such as cells, tissues, serum, and urine.

The Hydroxyproline Assay Kit may be used for:

• Cancer Research
• Cardiovascular Research
• Cosmetic Testing
• Dermatological Disease Research

The Hydroxyproline Assay Kit facilitates the measurement of hydroxyproline levels through a reaction where the oxidized form of hydroxyproline interacts with 4-(Dimethylamino)benzaldehyde (DMAB). This interaction produces a colorimetric response that can be measured at a wavelength of 560 nm, with the intensity of the color directly reflecting the concentration of hydroxyproline in the sample.

Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.

Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file