MAK613
Leucine Aminopeptidase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
LAP Activity Assay Kit
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About This Item
UNSPSC Code:
41116104
eCl@ss:
40050204
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
General description
Leucine aminopeptidase (LAP, EC 3.4.11.1) removes N-terminalleucine residues from peptides and proteins and is abundant in liver and intestinal tissues. LAP activity can increase in settings of tissue damage, tumorigenesis, or inflammation.
Application
For quantitative determination of LAP enzyme activity in biologicalsamples, such as cells, tissues, and serum samples.
For high-throughput screening of LAP inhibitors and evaluation of drug modulators.
For high-throughput screening of LAP inhibitors and evaluation of drug modulators.
Biochem/physiol Actions
The Leucine Aminopeptidase Activity Assay Kit provides a rapid colorimetric method in which LAP hydrolyzes a dye-linked leucine substrate to yield a yellow product measurable at 405 nm. The change in absorbance over 20 minutes is proportional to LAP activity. The linear detection range in 96-well plate is 0.025 to 250 U/L.
Storage Class
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
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