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MBD0032

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Eubacteria FISH probe - ATTO488

Probe for fluorescence in situ hybridization (FISH), 20 µM in water

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Quality Level

200

technique(s)

FISH: suitable

fluorescence

λex 504 nm; λem 521 nm (ATTO488)

shipped in

dry ice

storage temp.

−20°C

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells. Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.

FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood and tissue), microbial ecology (solid biofilms and aquatic systems) and plants.

Prokaryotic single cell life forms are divided into two domains, called Bacteria and Archaea, originally categorized as Eubacteria and Archaebacteria. However both terms, Eubacteria and Bacteria are still being used in microbiology. Eubacteria probe recognizes most bacteria as it is complementary to a portion of 16S rRNA found in almost all bacteria.,
FISH technique was successfully used to identify different bacteria with the universal bacterial probe in various samples such as, pure culture (as described in the figure legends), blood cultures,, periapical tooth lesions12, saliva13, biofilms from voice prostheses14, subgingival biofilm15, aortic wall tissue16, buccal epithelial cells, pure culture and cell culture17, intestine tissue embedded in paraffin18, necrotizing fasciitis and pure culture19, colon sections embedded in paraffin20,21, cancer tissues22,23, environmental samples24 and gut of the medicinal leech25. The probe can also be used for combined technique of FISH and Flow cytometric analysis. 9,26,27

It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative (MBD0034/35) control probes, that accompany the specific probe of interest.

Application

Eubacteria FISH probe - ATTO488 is suitable to use as a probe for fluorescence in situ hybridization (FISH) to recognize Eubacteria cells .

Features and Benefits

  • Visualize, identify and isolate bacteria cells.
  • Observe native bacteria cell populations in diverse microbiome environments.
  • Specific, sensitive and robust identification of bacteria cells in mixed microorganism population.
  • Specific, sensitive and robust identification even when bacteria are in low abundance in the sample.
  • FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
  • Provides information on bacteria morphology and allows to study biofilm architecture.
  • Identify various bacteria in environmental and clinical samples such as, formalin-fixed paraffin-embedded (FFPE) samples, blood cultures, saliva and more.
  • The ability to detect bacteria in its natural habitat is an essential tool for studying host-microbiome interaction.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Jiahui Yu et al.
International journal of cancer, 139(6), 1318-1326 (2016-05-01)
The prevalence of invasive Fusobacterium nucleatum (Fn) within the serrated neoplasia pathway of the proximal colon has seldom been investigated. We examined the invasive Fn and bacterial biofilms in 35 proximal hyperplastic polyps (HPs), 33 sessile serrated adenomas (SSAs), 48
K Trebesius et al.
Medical microbiology and immunology, 188(4), 169-175 (2000-08-05)
Fluorescence in situ hybridisation (FISH) targeted to ribosomal RNA is well established for studies in environmental microbiology. Initial applications of this technique in the field of medical microbiology showed that FISH is also a suitable means for the rapid, reliable
Claudia Schillinger et al.
PloS one, 7(5), e37583-e37583 (2012-06-02)
The polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. A number of these microorganisms have
J D Rudney et al.
Infection and immunity, 69(4), 2700-2707 (2001-03-20)
The mouth may provide an accessible model for studying bacterial interactions with human cells in vivo. Using fluorescent in situ hybridization and laser scanning confocal microscopy, we found that human buccal epithelial cells from 23 of 24 subjects were infected
Francesca Cavrini et al.
Journal of medical microbiology, 54(Pt 1), 93-96 (2004-12-14)
Treponema denticola and Porphyromonas gingivalis have been identified in atheromatous plaques of two patients suffering from atherosclerosis by PCR and fluorescence in situ hybridization (FISH). The use of the FISH technique suggested that these periodontopathic micro-organisms might be metabolically active
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