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MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Synonym(s):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Product Name

Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes
  • HepG2 cells

Features and Benefits

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Other Notes

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS

  • C0356Protein Extraction Reagent Type 4 1 bottleSDS

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Regulatory Information

低风险生物材料
常规特殊物品
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Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria
Roundhill, EA and Burchill, SA
British Journal of Cancer, 106(6), 1224-1224 (2012)
Imran Khan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 789-799 (2016-10-11)
Although quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3'-dihydroxy-3, 7, 4'-triethoxyflavone), a newly synthesized quercetin derivative on HCT-116 colon cancer cells. After 24h of
Linezolid trough concentrations correlate with mitochondrial toxicity-related adverse events in the treatment of chronic extensively drug-resistant tuberculosis
Song T, et al.
EBioMedicine, 2(11), 1627-1633 (2015)
The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain
Fossati S, et al.
Neurobiology of Disease, 86, 29-40 (2016)
Mitochondria
Cooper G M
Journal of biological and chemical chronicles (2000)

Articles

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

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