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MRN10

Sigma-Aldrich

GenElute™ mRNA Miniprep Kit

sufficient for 10 purifications

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Synonym(s):
GenElute™ mRNA Kit, Gen Elute
NACRES:
NA.52

usage

sufficient for 10 purifications

Quality Level

200

technique(s)

RNA purification: suitable

storage temp.

15-25°C

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General description

Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.

Application

The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.

Features and Benefits

  • Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
  • Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
  • One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells

Other Notes

For additional information, please see www.sigma-aldrich.com/mrna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Jianzi Lan et al.
Cellular & molecular biology letters, 27(1), 51-51 (2022-06-28)
Diabetic nephropathy (DN) is prevalent in patients with diabetes. N6-methyladenosine (m6A) methylation has been found to cause modification of nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing (NLRP) 3, which is involved in cell pyroptosis and inflammation. WTAP is a
Gang Luo et al.
Foods (Basel, Switzerland), 11(11) (2022-06-11)
N6-methyladenosine (m6A) is the most prevalent internal mRNA modification in eukaryotes. The M6A modification plays an important role in transcription and cell function. The mechanism by which m6A modification regulates meat quality remains elusive. In this study, gene knockout and
Liping Wu et al.
Frontiers in immunology, 13, 839677-839677 (2022-06-28)
Host translation is generally modulated by viral infection, including duck hepatitis A virus (DHAV) infection. Previously, we reported that cellular protein synthesis in a cell model of duck embryo fibroblasts is significantly inhibited by DHAV infection but not viral proteins
Caitlin M A Simopoulos et al.
G3 (Bethesda, Md.), 9(8), 2511-2520 (2019-06-27)
Long non-coding RNAs (lncRNAs) represent a diverse class of regulatory loci with roles in development and stress responses throughout all kingdoms of life. LncRNAs, however, remain under-studied in plants compared to animal systems. To address this deficiency, we applied a
Caitlin M A Simopoulos et al.
BMC genomics, 21(1), 396-396 (2020-06-10)
The severity and frequency of drought has increased around the globe, creating challenges in ensuring food security for a growing world population. As a consequence, improving water use efficiency by crops has become an important objective for crop improvement. Some
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