MSANGERV
Sanger Arrayed Whole Genome Lentiviral CRISPR Library
Mouse, Virus Format
Synonym(s):
Arrayed CRISPR library, Sanger CRISPR library
Quality Level
packaging
pkg of 10 μL (384-well plate)
concentration
1x106 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
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General description
Application
Features and Benefits
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
Additional Features
- Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
Packaging
Physical form
Other Notes
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Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Articles
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在,每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量,可确保尽可能特异性的筛选,同时控制时间和成本。
获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。
Protocols
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
了解桑格测序步骤或链终止法、DNA 测序的工作原理以及如何在研究中准确读取桑格测序结果。
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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