MSKT0004
IA-MS Immunoaffinity Mass Spectrometry Kit - Protein A/G
96 Well Strip Plate with SOLu-Trypsin
Synonym(s):
Target protein enrichment and quantification from complex background by mass spectrometry.
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About This Item
UNSPSC Code:
41115859
NACRES:
NA.32
form
solid
suitability
suitable for mass spectrometry
shipped in
wet ice
storage temp.
2-8°C
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General description
The Protein A/G IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own capture antibody and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.
Application
Target protein enrichment and quantification from complex background by mass spectrometry.
Features and Benefits
- Robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS
- Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
- 96-well plate format has potential for automation in a liquid-handling system
- Bead-free system circumvents messy clean-up and eliminates risk of column clogging
- Plate strips allow user to run as many or as few samples as desired
Packaging
Box with foam insert containing kit components.
Preparation Note
2 years when stored at 2-8 °C.
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Other Notes
Protein A/G Coated Plates SAE0113-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce™ plate seal Z721581-1EA
See datasheet for more information.
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce™ plate seal Z721581-1EA
See datasheet for more information.
Legal Information
EZ-Pierce is a trademark of Excel Scientific, Inc.
TWEEN is a registered trademark of Croda International PLC
Kit Components Only
Product No.
Description
- MS Denaturation Solution
- Rapid Trypsin Digestion Buffer
Kit Components Also Available Separately
Product No.
Description
SDS
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
75.2 °F
Flash Point(C)
24 °C
Regulatory Information
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Instructions
Instructions
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