SILu^™Lite MAPK3 Mitogen activated protein kinase 3 human

SILu^™ Lite MAPK3 is a recombinant human protein expressed in human 293 cells. It is a monomer of 399 amino acids (including N-terminal polyhistidine and flag tags), with a molecular weight of ~45.5 kDa. SILu^™ Lite MAPK3 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.

MAPK3, the first mammalian MAPK to be characterized and cloned, is commonly expressed in most tissues and is activated through the small guanosine triphosphatase Ras and sequential activation of the protein kinases Raf and MEK upon stimulation of cells with a broad range of extracellular signals. Erk was suggested to be a good marker for estradiol and tamoxifen effects. Alzheimer′s disease′s Erk1 and Erk2 index values were inversely correlated with disease duration, suggesting maximal efficacy for early diagnosis. In addition, differential Erk1 and Erk2 phosphorylation, could have important clinical utility for providing increased certainty in the positive diagnosis of AD, particularly in the early phase of disease progression.

Supplied as a 0.1 mg/mL solution of 20mM sodium phosphate, pH 8.0, 1M NaCl.

MDYKDDDDKGHHHHHHHHGGQAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP

SILu is a trademark of Sigma-Aldrich Co. LLC

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