MTOX1092
MRP1 Knockout Caco-2 Cells
human male colorectal tissue (Source Disease: colon adenocarcinoma)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32039090
Biological source:
human male colorectal tissue (Source Disease: colon adenocarcinoma)
Product Name
MRP1 Knockout Caco-2 Cells, one vial
biological source
human male colorectal tissue (Source Disease: colon adenocarcinoma)
Quality Segment
form
liquid
technique(s)
drug transporter assay: suitable, permeability assay: suitable
application(s)
ADME/TOX
storage temp.
−196°C
Gene Information
human ... ABCC1(4363)
General description
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MRP1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCC1 (MRP1) efflux transporter.
Application
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Features and Benefits
The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
- A functional knockout of the MRP1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP1 substrate
- The vial format enables the MRP1 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
Legal Information
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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