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Merck
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MTOXRCSUP

RPTEC Complete Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

Synonym(s):

Basal media supplement for huRPTEC

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

Key Documents

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Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

Related Categories

General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Application

RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.

Preparation Note

Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--人源产品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
0000360526
0000301513
0000301513
0000360526
SLCM2566

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Development and application of human renal proximal tubule epithelial cells for assessment of compound toxicity.
Li S, et al.
Current Chemical Genomics and Translational Medicine, 11, 19-19 (2017)
Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs

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Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters and Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.

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