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MWGF200

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

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Synonym(s):
Gel Filtration Protein Ladder
NACRES:
NA.25

form

solid

Quality Level

200

technique(s)

liquid chromatography (LC): suitable

storage temp.

−20°C

General description

Gel filtration markers kit contains five vials of proteins and 1 vial of blue dextran for calibration.

Application

Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS
  • A8531Albumin, bovine serum, 66,000 Da MW 1 mL/vialSDS
  • A8656Alcohol Dehydrogenase, yeast, 150,000 Da MW 1 mL/vialSDS
  • A8781β-Amylase, sweet potato, 200,000 Da MW 1 mL/vialSDS
  • D4772 Blue dextran, 2,000,000 Da MW 1 mL/vialSDS
  • C7025Carbonic Anhydrase, bovine erythrocytes 29,000 Da MW 1 mL/vialSDS
  • C7150Cytochrome c, horse heart, 12,400 Da MW 1 mL/vialSDS

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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T1503
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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Daisuke Seo et al.
Biochimica et biophysica acta. Bioenergetics, 1861(3), 148140-148140 (2019-12-16)
Among the thioredoxin reductase-type ferredoxin-NAD(P)+ oxidoreductase (FNR) family, FNR from photosynthetic purple non‑sulfur bacterium Rhodopseudomonas palustris (RpFNR) is distinctive because the predicted residue on the re-face of the isoalloxazine ring portion of the FAD prosthetic group is a tyrosine. Here
Andreas Krug et al.
The Journal of biological chemistry, 280(1), 585-595 (2004-10-21)
In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type
Cristina L Marolda et al.
Journal of bacteriology, 190(6), 2128-2137 (2008-01-22)
Wzz is a membrane protein that determines the chain length distribution of the O-antigen lipopolysaccharide by an unknown mechanism. Wzz proteins consist of two transmembrane helices separated by a large periplasmic loop. The periplasmic loop of Escherichia coli K-12 Wzz
Ulrich Strych et al.
BMC microbiology, 7, 40-40 (2007-05-19)
Over the past fifteen years, antibiotic resistance in the Gram-positive opportunistic human pathogen Streptococcus pneumoniae has significantly increased. Clinical isolates from patients with community-acquired pneumonia or otitis media often display resistance to two or more antibiotics. Given the need for
Suguru Oguri et al.
Bioscience, biotechnology, and biochemistry, 72(10), 2640-2650 (2008-10-08)
A cDNA encoding tomato fruit lectin was cloned from an unripe cherry-tomato fruit cDNA library. The isolated lectin cDNA contained an open reading frame encoding 365 amino acids, including peptides that were sequenced. The deduced sequence consisted of three distinct
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