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Merck
CN

MWGF70

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da

Synonym(s):

Gel Filtration Protein Ladder

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25
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form

solid

technique(s)

protein quantification: suitable

storage temp.

−20°C

General description

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins. Fractionation is based on the diffusion of molecules into the pores of the resin. Larger proteins do not enter the pores of the resin as readily but pass through the fluid volume of the column faster than smaller proteins. These protein molecules elute from the column in order of decreasing molecular mass.

Application

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da has been used as a standard in size exclusion chromatography.
Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS

  • A8531Albumin, Bovine serum, 66,000 MW 1 mL/vialSDS

  • A3886Aprotinin, Bovine lung, 6,500 MW 1 mL/vialSDS

  • D4772Blue Dextran 2,000,000 MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, Bovine erythrocytes, 29,000 MW 1 mL/vialSDS

  • C7150Cytochrome c, Horse heart, 12,400 MW 1 mL/vialSDS

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Information

监管及禁止进口产品
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Purification and some properties of Mn peroxidase from <I>Lentinula edodes</I>.
Boer, C.G., et al.
Process. Biochem., 41(5), 1203-1207 (2006)
Kok Hong Lim et al.
Biochemistry, 50(40), 8682-8691 (2011-09-07)
Although streptavidin's high affinity for biotin has made it a widely used and studied binding protein and labeling tool, its tetrameric structure may interfere with some assays. A streptavidin mutant with a simpler quaternary structure would demonstrate a molecular-level understanding
Miriam Kuester et al.
Journal of molecular biology, 409(2), 189-201 (2011-04-06)
Death receptors belong to the tumor necrosis factor receptor (TNFR) super family and are intimately involved in the signal transduction during apoptosis, stress response and cellular survival. Here we present the crystal structure of recombinantly expressed death receptor six (DR6)
Cristina Cocco et al.
Journal of anatomy, 217(6), 683-693 (2010-11-03)
VGF mRNA and its precursor-derived products are selectively expressed in certain neurons and promptly respond to neurotrophins and to neural/electrical activity. Proteomic studies have previously revealed a reduction in some VGF peptides in the cerebrospinal fluid of patients affected by
Yu Chih Liu et al.
Protein expression and purification, 107, 29-34 (2014-12-03)
Helicobacter pylori infections are associated with gastritis, duodenal and gastric ulcers and gastric adenocarcinoma. Bacterial chemotaxis, mediated by four different chemoreceptors (also termed transducer-like proteins (Tlp)), plays an important role in initial colonization and development of disease. Chemoreceptor sensory domains

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