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N1290

Polynucleotide phosphorylase human

Synonym(s):

Polyribonucleotide nucleotidyltransferase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
2.7.7.8 (BRENDA, IUBMB)
MDL number:
MFCD00131992
Recombinant:
expressed in E. coli
Concentration:
400-600 μg/mL protein
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recombinant

expressed in E. coli

form

solution

enzyme activity

≥20 units/mg protein

mol wt

~90 kDa

concentration

400-600 μg/mL protein

shipped in

dry ice

storage temp.

−70°C

Biochem/physiol Actions

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.

Physical form

supplied as a solution in 20 mM HEPES buffer, pH 7.9, with 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, ~130 mM KCl, and 20% (w/v) glycerol.

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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