N1290
Polynucleotide phosphorylase human
Synonym(s):
Polyribonucleotide nucleotidyltransferase
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About This Item
Recombinant:
expressed in E. coli
Concentration:
400-600 μg/mL protein
recombinant
expressed in E. coli
form
solution
enzyme activity
≥20 units/mg protein
mol wt
~90 kDa
concentration
400-600 μg/mL protein
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Physical form
supplied as a solution in 20 mM HEPES buffer, pH 7.9, with 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, ~130 mM KCl, and 20% (w/v) glycerol.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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