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N1408

Nerve Growth Factor β Human

Name: Nerve Growth Factor β Human
Brand: SIGMA

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

HSAN5, NGF-β human, NGFB, hNGFβ

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00164982

Biological source:

human

Recombinant:

expressed in NSO cells

Assay:

>97% (SDS-PAGE)

Form:

lyophilized powder

Impurities:

<1 EU/μg

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Properties

Product Name

NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in NSO cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.15-3.0 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

packaging

pkg of 5X0.1 mg, pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

solubility

water: soluble 0.1 g/L at 20 °C

UniProt accession no.

P01138

storage temp.

−20°C

Gene Information

human ... NGF(4803)

Description

General description

Recombinant nerve growth factor β (NGFB) is supplied as a lyophilized protein for use in mammalian cell culture research involving neuronal development and differentiation studies in vitro.

Application

NGF-β human has been used:

in protein pin arrays
to induce U87-MG (glioblastoma cell line) cell proliferation
in drug release kinetics

Biochem/physiol Actions

Nerve Growth Factor‑β (NGF‑β) is a neurotrophic factor that mediates cellular responses related to growth, differentiation, and survival of neuronal and non-neuronal cells. NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Physical form

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Analysis Note

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c