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Merck
CN

N1531

Denaturation Solution

for Northern and Southern blotting

Synonym(s):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

Key Documents

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Product Name

Denaturation Solution, for Northern and Southern blotting

grade

Molecular Biology

form

solution

storage temp.

room temp

Quality Level

200

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Other Notes

0.5 N NaOH, 1.5 M NaCl
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000489811
0000489811
0000445851
0000445851
0000438535

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José Manuel Pérez-Pérez et al.
Plant physiology, 134(1), 101-117 (2004-01-20)
The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Related Content

Data Sheet - N1531

Instructions

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