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N1665

Sigma-Aldrich

5′-Nucleotidase human

recombinant, expressed in CHO cells, vial of 6-12 μg

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Synonym(s):
CD73, NT5E, ecto-5′-nucleotidase
CAS Number:
9027-73-0
Enzyme Commission number:
3.1.3.5 (BRENDA, IUBMB)
MDL number:
MFCD00130311
NACRES:
NA.54

recombinant

expressed in CHO cells

Quality Level

200

description

contains a C-terminal 6-His tag

Assay

≥90% (SDS-PAGE)

form

solution

enzyme activity

≥15 units/mg protein

mol wt

~61 kDa by SDS-PAGE (reducing)

packaging

vial of 6-12 μg

impurities

≤1.0 EU/μg endotoxin

NCBI accession no.

NP_002517

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... NT5E(4907)

General description

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

Biochem/physiol Actions

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.
5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

Unit Definition

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

Physical form

Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
F W Sunderman
Annals of clinical and laboratory science, 20(2), 123-139 (1990-03-01)
This review delineates the subcellular distribution, biochemical characteristics, and metabolic functions of 5'-nucleotidase (5'NT), summarizes the analytical biochemistry of 5'NT, and assesses the clinical significance of 5'NT determinations in body fluids, cells, and tissues. Salient aspects of the clinical biochemistry
Paul A Beavis et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14711-14716 (2013-08-22)
CD73 inhibits antitumor immunity through the activation of adenosine receptors expressed on multiple immune subsets. CD73 also enhances tumor metastasis, although the nature of the immune subsets and adenosine receptor subtypes involved in this process are largely unknown. In this
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