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N2250

Naphthol AS-BI phosphate disodium salt hydrate

fluorogenic, ≥96.0% (HPLC), powder

Synonym(s):

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

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About This Item

Empirical Formula (Hill Notation):
C18H13BrNNa2O6P · xH2O
CAS Number:
207569-04-8
Molecular Weight:
496.16 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
24897558
NACRES:
NA.83
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Product Name

Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)

Quality Level

200

assay

≥96.0% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O

InChI

1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2

InChI key

WNPKMQCBZKBNOS-UHFFFAOYSA-L

Related Categories

General description

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.
Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6231
BCCM7980
BCCH6248
BCCD0900
BCBL3289V

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Establishment of human cementifying fibroma cell lines by transfection with temperature-sensitive simian virus-40 T-antigen gene and hTERT gene
Kudo Y, et al.
Bone, 30(5), 712-717 (2002)
Enhanced fibrillin-2 expression is a general feature of wound healing and sclerosis: potential alteration of cell attachment and storage of TGF-beta
Brinckmann J, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 90(5), 739-752 (2010)
Naphthol AS-BI (7-bromo-3-hydroxy-2-naphtho-o-anisidine) phosphatase and naphthol AS-BI beta-D-glucuronidase in Chinese hamster ovary cells: biochemical and flow cytometric studies.
Dolbeare FA and Phares W
The Journal of Histochemistry and Cytochemistry, 27(1), 120-124 (1979)
Effects of estrogen deficiency on microstructural changes in rat alveolar bone proper and periodontal ligament
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Cells, 10(3) (2021-04-04)
Older age is associated with reduced immune function. Our aim was to study how age affects the development of apical periodontitis (AP). AP was induced in two age groups of mice (young vs. adult). Histological samples were stained by Hematoxylin

Global Trade Item Number

SKUGTIN
N2250-1G04061834110279
N2250-5G04061834110286
N2250-100MG04061833273098
N2250-50MG04061833273111
N2250-500MG04061833273104

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