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N2250

Naphthol AS-BI phosphate disodium salt hydrate

Name: Naphthol AS-BI phosphate disodium salt hydrate
Brand: SIGMA

fluorogenic, ≥96.0% (HPLC), powder

Synonym(s):

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

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About This Item

Empirical Formula (Hill Notation):

C₁₈H₁₃BrNNa₂O₆P · xH₂O

CAS Number:

207569-04-8

Molecular Weight:

496.16 (anhydrous basis)

UNSPSC Code:

12352204

PubChem Substance ID:

24897558

NACRES:

NA.83

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Properties

Product Name

Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)

Quality Segment

200

assay

≥96.0% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O

InChI

1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2

InChI key

WNPKMQCBZKBNOS-UHFFFAOYSA-L

Description

General description

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:

in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
N2250-5G	04061834110286
N2250-1G	04061834110279
N2250-100MG	04061833273098
N2250-50MG	04061833273111
N2250-500MG	04061833273104