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N2415

Nucleoside Phosphorylase bacterial

Name: Nucleoside Phosphorylase bacterial
Brand: SIGMA

recombinant, expressed in E. coli, ≥10 units/mg protein

Synonym(s):

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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About This Item

CAS Number:

9030-21-1

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-857-3

MDL number:

MFCD00131739

EC Number:

2.4.2.1 (BRENDA, IUBMB)

Specific activity:

≥10 units/mg protein

Assay:

≥80% protein basis (biuret)

Recombinant:

expressed in E. coli

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recombinant

expressed in E. coli

Quality Level

200

assay

≥80% protein basis (biuret)

form

lyophilized powder

specific activity

≥10 units/mg protein

sequence note

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

UniProt accession no.

P0ABP8

storage temp.

−20°C

SMILES string

[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

Gene Information

Escherichia coli ... deoD(945654)

Application

Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Biochem/physiol Actions

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.

Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO₄, pH 7.4 solution.

Other Notes

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

0000492355

0000488017

0000466004

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A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida.

Patricia Calero et al.

Nature communications, 11(1), 5045-5045 (2020-10-09)

Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and

Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders

Johnston S B and Raines R T

Biochemistry, 54(7), 1576-1582 (2015)

Primary T-cell immunodeficiencies

Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)

Synthesis of α-D-Ribose 1-Phosphate and 2-Deoxy-α-D-Ribose 1-Phosphate Via Enzymatic Phosphorolysis of 7-Methylguanosine and 7-Methyldeoxyguanosine.

Irina V Varizhuk et al.

Current protocols, 2(1), e347-e347 (2022-01-21)

A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis

Disorders of purines and pyrimidines

Handbook of Clinical Neurology, 827-838 (2014)

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Global Trade Item Number

SKU	GTIN
N2415-250UN	04061832924694
N2415-1KU	04061826691885
N2415-100UN	04061832924687

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