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Merck
CN

N3262

NCTC 135 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

NACRES:
NA.75
UNSPSC Code:
12171500

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Product Name

NCTC 135 Medium, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

shipped in

ambient

storage temp.

2-8°C

Related Categories

Application

NCTC 135 Medium has been used:
  • to culture Philophthalmus sp. rediae in vitro
  • to culture Ehrlich ascites mouse tumor cell line
  • as a culture media for the differentiation of Rcho-1 cells

General description

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000448676
0000448672
0000448672
0000448676
25B299

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Synthesis, characterization and antitumor activity of 1, 2-disubstituted ferrocenes and cyclodextrin inclusion complexes
Petrovski Z, et al.
Journal of Organometallic Chemistry, 693, 675-684 (2008)
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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