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N3393

Naphthol Blue Black

Name: Naphthol Blue Black
Brand: SIGMA

BioReagent, suitable for electrophoresis

Synonym(s):

Acid Black 1, Amido Black 10B, Buffalo Black NBR

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About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄N₆Na₂O₉S₂

CAS Number:

1064-48-8

Molecular Weight:

616.49

EC Number:

213-903-1

UNSPSC Code:

41105322

NACRES:

NA.32

PubChem Substance ID:

24897642

MDL number:

MFCD00004017

Beilstein/REAXYS Number:

5374263

Colour Index Number:

20470

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product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ~85%

technique(s)

protein staining: suitable

impurities

≤10% water

suitability

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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Related Categories

Protein Electrophoresis & Western Blotting

Protein Electrophoresis Gel Stains

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1B

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.

Meyer D, et al.

Journal of Bacteriology, 188, 4903-4917 (2006)

Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.

E De Vito et al.

Experientia, 22(2), 124-125 (1966-02-15)

Identification of a gene responsible for amido black decolorization isolated from Amycolatopsis orientalis.

M D Chengalroyen et al.

World journal of microbiology & biotechnology, 29(4), 625-633 (2012-11-29)

Investigation into the biodegradative capability of several actinomycetes led to the discovery of many strains possessing an ability to decolorize a variety of azo and triphenylmethane dyes. Of particular interest is an Amycolatopsis sp. isolate which displayed the ability to

Comparative study on the degradation of I.C. Remazol Brilliant Blue R and I.C. Acid Black 1 by Fenton oxidation and Fe 0/air process and toxicity evaluation.

Shih-Hsien Chang et al.

Journal of hazardous materials, 166(2-3), 1279-1288 (2009-01-23)

Degradation of Remazol Brilliant Blue R (anthraquinone dye) and Acid Black 1 (azo dye) by Fenton oxidation and low-cost Fe(0)/air process was compared. The doses of Fenton reagent needed for decolorization of Remazol Brilliant Blue R was much higher than

Regulation of gene expression in Neurospora crassa with a copper responsive promoter.

Teresa M Lamb et al.

G3 (Bethesda, Md.), 3(12), 2273-2280 (2013-10-22)

Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled

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Global Trade Item Number

SKU	GTIN
N3393-100G	04061834116011
N3393-25G	04061834116028

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