Skip to Content
Merck
CN

Key Documents

View All Documentation

N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

Synonym(s):

Acid Black 1, Amido Black 10B, Buffalo Black NBR

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
1064-48-8
Molecular Weight:
616.49
EC Number:
213-903-1
UNSPSC Code:
41105322
NACRES:
NA.32
PubChem Substance ID:
24897642
MDL number:
MFCD00004017
Beilstein/REAXYS Number:
5374263
Colour Index Number:
20470
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

product line

BioReagent

Quality Segment

200

form

powder

composition

Dye content, ~85%

technique(s)

protein staining: suitable

impurities

≤10% water

suitability

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

Still not finding the right product?

Explore all of our products under Naphthol Blue Black

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1B

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library