N3665
Pyrimidine Nucleoside Phosphorylase from Bacillus subtilis
recombinant, expressed in E. coli, ≥70 units/mg protein
Synonym(s):
PynP
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recombinant
expressed in E. coli
Quality Segment
form
lyophilized powder
specific activity
≥70 units/mg protein
storage temp.
−20°C
General description
The roles of the residues in the catalytic active site of pyrimidine nucleoside phosphorylase from Bacillis subtilis have been elucidated using hybrid quantum-mechanical/molecular-mechanical methods.
Biochem/physiol Actions
Pyrimidine nucleoside phosphorylase functions in the nucleotide synthesis salvage pathway by catalyzing the reversible phosphorolysis of pyrimidines. Both uridine and thymidine are substrates.
Other Notes
One unit will convert 1 μmole each of 2′-deoxyuridine and phosphate to uracil and 2-deoxyribose 1-phosphate per minute at pH 7.4 and 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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