N4787
Nek2, Active human
recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
Never In Mitosis Gene A-Related Kinase 2, Nima-Related Kinase 2
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About This Item
Assay:
≥85% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
assay
≥85% (SDS-PAGE)
form
buffered aqueous glycerol solution
enzyme activity
≥10 units/mg protein
mol wt
protein apparent mol wt ~80 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEK2(4751)
Application
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
Biochem/physiol Actions
Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
Other Notes
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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