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Merck
CN

N4887

Newborn Calf Serum

USA origin, γ-irradiated, sterile-filtered, suitable for cell culture

Synonym(s):

NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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About This Item

UNSPSC Code:
12352207
MDL number:
Technical Service
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biological source

bovine (calf)

sterility

γ-irradiated, sterile-filtered

origin

USA origin

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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Application

Newborn calf serum (NCS) is collected within 10 days post partum calves. NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.

Preparation Note

Collected from calves that are 10 days old or less.
Exposed to 25-35 kGy of irradiation.

Analysis Note

Endotoxin tested

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

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Ling Chen et al.
American journal of nephrology, 25(5), 459-465 (2005-08-25)
Oxidative stress has been considered to be a common pathogenetic factor of diabetic nephropathy. But the reason why renal cells are susceptible to oxidative injury in diabetes is not clear. Vitamin C plays a central role in the antioxidant defense
Kyoko Shimizu et al.
Cell biology international, 30(4), 381-388 (2006-03-15)
We have recently developed a primary culture system for visceral adipocytes (VAs) using stomal-vascular cells (SVCs) isolated from the mesenteric fat tissue of male Sprague-Dawley rats of 3-5 weeks of age. Modified Dulbecco's modified Eagle medium (DMEM)/F12 containing 17 microM
R Kishida et al.
Theriogenology, 62(9), 1663-1676 (2004-10-30)
To establish a defined in vitro maturation culture system for porcine oocytes, we examined the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation medium. Furthermore, to evaluate cytoplasmic maturation, we investigated GSH concentrations and embryo
Davide Camposampiero et al.
Cornea, 22(3), 254-258 (2003-03-27)
To explore the possibility of replacing bovine serum with chicken ovalbumin in cornea preservation. Twenty-one pairs of corneas were cultivated at 31 degrees C in a medium containing either 2% (vol/vol) of newborn calf serum (one cornea) or 2 mg/mL

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