N5264
Monoclonal Anti-Neurofilament 160 antibody produced in mouse
clone NN18, ascites fluid
Synonym(s):
Monoclonal Anti-Neurofilament medium chain
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NN18, monoclonal
mol wt
antigen 160 kDa
contains
15 mM sodium azide
species reactivity
human, pig
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections
immunohistochemistry (frozen sections): suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified, unmodified
Gene Information
chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
General description
Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins.
Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.
Immunogen
neurofilaments from pig spinal cord.
Application
Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Monoclonal anti-neurofilament 160 antibody has been used in
- Immunoblotting.
- Immunohistochemistry
- Immunofluorescence
Biochem/physiol Actions
Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.
Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Svetlana Gorokhova et al.
PLoS genetics, 10(2), e1004081-e1004081 (2014-02-12)
Neurotrophins and their receptors control a number of cellular processes, such as survival, gene expression and axonal growth, by activating multiple signalling pathways in peripheral neurons. Whether each of these pathways controls a distinct developmental process remains unknown. Here we
F Maina et al.
Genes & development, 11(24), 3341-3350 (1998-02-07)
The development of the nervous system is a dynamic process during which factors act in an instructive fashion to direct the differentiation and survival of neurons, and to induce axonal outgrowth, guidance to, and terminal branching within the target tissue.
Tiziana Bisogno et al.
The Journal of cell biology, 163(3), 463-468 (2003-11-12)
Diacylglycerol (DAG) lipase activity is required for axonal growth during development and for retrograde synaptic signaling at mature synapses. This enzyme synthesizes the endocannabinoid 2-arachidonoyl-glycerol (2-AG), and the CB1 cannabinoid receptor is also required for the above responses. We now
M K Lee et al.
Current opinion in cell biology, 6(1), 34-40 (1994-02-01)
Neurofilaments make up the major intermediate filament system in mature neurons. Recent studies demonstrate that neurofilaments in vivo are obligate heteropolymers and are required for proper radial growth of axons. Furthermore, forced over-expression of neurofilament subunits in transgenic mice shows
Hak Hyun Ryu et al.
Journal of veterinary science, 10(4), 273-284 (2009-11-26)
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by
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