N5386
Nuclease micrococcal from Staphylococcus aureus
100-300 units/mg protein
Synonym(s):
Endonuclease micrococcal, MNase, Micrococcal endonuclease
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
Specific activity:
100-300 units/mg protein
Biological source:
Staphylococcus aureus
biological source
Staphylococcus aureus
Quality Segment
form
powder
specific activity
100-300 units/mg protein
composition
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
technique(s)
DNA extraction: suitable, DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
−20°C
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
Application
Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.
Biochem/physiol Actions
Hydrolyzes 5′-phosphodiester bonds of DNA.
Other Notes
Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
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QC Methods
Global Trade Item Number
| SKU | GTIN |
|---|---|
| N5386-50UN | 04061832758534 |
| N5386-200UN | 04061832758510 |
| N5386-500UN | 04061832758527 |