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N5632

Nile Blue A

Name: Nile Blue A
Brand: SIGMA

Dye content ≥75 %, Powder

Synonym(s):

Basic Blue 12, Nile blue sulfate

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About This Item

Linear Formula:

2C₂₀H₂₀N₃O · SO₄

CAS Number:

3625-57-8

Molecular Weight:

732.85

NACRES:

NA.47

PubChem Substance ID:

24897743

UNSPSC Code:

12171500

EC Number:

222-832-5

MDL number:

MFCD00064529

Colour Index Number:

51180

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Product Name

Nile Blue A, Dye content ≥75 %

form

powder

Quality Level

100

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

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Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Segmental origin and migration of neural crest cells in the hindbrain region of the chick embryo.

Lumsden A

Development, 113, 1281-1281 (1991)

Fgfr1 regulates patterning of the pharyngeal region.

Trokovic N

Genes & Development, 17, 141-141 (2003)

Biochemical, histochemical and ultrastructural characterization of Centrolobium robustum (Fabaceae) seeds.

Pinzon-Torres JA

Hoehnea, 36, 149-149 (2009)

Rost FWD

Fluorescence Microscopy (1992)

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A.

M Oshiki et al.

Water science and technology : a journal of the International Association on Water Pollution Research, 64(3), 747-753 (2011-11-22)

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated

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Global Trade Item Number

SKU	GTIN
N5632-25G	04061834117117

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