N5661
Nuclease S1 from Aspergillus oryzae
for single-strand DNA/RNA digestion
Synonym(s):
Endonuclease S1
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Biological source:
Aspergillus sp. (A. oryzae)
Concentration:
≥100000 units/mL
biological source
Aspergillus sp. (A. oryzae)
Quality Segment
form
solution
concentration
≥100000 units/mL
technique(s)
DNA purification: suitable
input
plant, fungi and insect sample(s)
suitability
suitable for nucleic acid purification
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.
Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.
Physical form
Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein
Other Notes
One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.
Kit Components Only
Product No.
Description
- 30mM Sodium acetate .25-.25 %
- 50mM Sodium chloride .29 %
- 1mM Zinc chloride .01 %
- Glycerol 50 %
- 2mg/mL Protein .2 %
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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