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Merck
CN

N6635

Nutrient Mixture F-10 Ham

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-10

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217607

Key Documents

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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues.

Application

Nutrient Mixture F-10 Ham has been used:

  • for the in vitro tissue culture from rabbit
  • to culture satellite cells from fish white muscle of trout
  • for culturing neonatal porcine islets (NPIs)

Preparation Note

Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000502092
0000502092
0000500474
0000500474
0000500476

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Amirhosein Hasani et al.
Life sciences, 254, 117767-117767 (2020-05-15)
Heat stress shock affects the generation of free radicals and can have a harmful effect on spermatogenesis. Photobiomodulation (PBM) is very effective in andrology for treating male infertility. This research aimed at the evaluation of the impacts of PBM on
M A Silvestre et al.
Theriogenology, 58(1), 69-76 (2002-08-17)
Vitrification (3.58 M EG and 2.82 M DMSO in PBS with 20% FCS) and rapid-freezing (0.25 M sucrose, 2.25 M EG, and 2.25 M DMSO in PBS with 20% FCS) procedures were assayed to cryopreserve rabbit tissue samples from 12-day
Fernanda de Mello et al.
General and comparative endocrinology, 210, 23-29 (2014-12-03)
Members of the TGF-β superfamily are involved in numerous cell functions; however, except for myostatin, their roles in the regulation of muscle growth in fish are completely unknown. We measured tgf-β1, tgf-β2, tgf-β3, inhibin βA (inh) and follistatin (fst) gene
Azar Afshar et al.
Reproductive sciences (Thousand Oaks, Calif.), 28(2), 371-380 (2020-08-12)
Spermatogenesis process is sensitive to heat stress because the testicular temperature is 2 to 4 °C lower than the core body temperature. The current study aimed to investigate the effects of iron oxide nanoparticles containing curcumin on spermatogenesis in mice induced
Ensieh Sajadi et al.
Journal of cellular biochemistry, 120(10), 17312-17325 (2019-05-22)
The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three-dimensional pattern of the testis
View All Related Papers

Articles

Nutrient Mixture F-10 Ham Formulation

Nutrient Mixture F-10 Ham Formulation

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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