Neuraminidase from Vibrio cholerae

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Chromatographically purified.

Preservative free.

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.