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N8016

2-Nitrophenyl β-D-glucopyranoside

Name: 2-Nitrophenyl &#946;-<SC>D</SC>-glucopyranoside
Brand: SIGMA

≥99% (TLC), powder

Synonym(s):

2-Nitrophenyl-beta-D-glucopyranoside, o-NPG

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About This Item

Empirical Formula (Hill Notation):

C₁₂H₁₅NO₈

CAS Number:

2816-24-2

Molecular Weight:

301.25

NACRES:

NA.28

PubChem Substance ID:

24897848

UNSPSC Code:

12352204

EC Number:

220-568-5

MDL number:

MFCD00065048

Beilstein/REAXYS Number:

92206

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Properties

Product Name

2-Nitrophenyl β-D-glucopyranoside, powder

assay

≥99% (TLC)

Quality Level

300

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-RMPHRYRLSA-N

Description

Application

2-Nitrophenyl β-D-glucopyranoside has been used as a substrate for glucosidase in protein assay and in a colorimetric assay to determine protein stability.

Biochem/physiol Actions

2-Nitrophenyl β-D-glucopyranoside serves as an artificial substrate for glucosidase.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000542312

0000483140

0000439943

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Articles

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Ion implantation treatment of beads for covalent binding of molecules: application to bioethanol production using thermophilic beta-glucosidase.

Neil J Nosworthy et al.

Enzyme and microbial technology, 54, 20-24 (2013-11-26)

We have achieved plasma immersion ion implantation (PIII) treatment of beads and powders using a specially designed plasma treatment system. This simple one-step production of functionalized beads provides an attractive alternative to current commercial functional beads, for which proteins must

Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution.

J H Lebbink et al.

Biochemistry, 39(13), 3656-3665 (2000-03-29)

The beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (CelB) is the most thermostable and thermoactive family 1 glycosylhydrolase described to date. To obtain more insight in the molecular determinants of adaptations to high temperatures and study the possibility of optimizing

Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.

M Moracci et al.

Biochemistry, 37(49), 17262-17270 (1998-12-23)

The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst.

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Global Trade Item Number

SKU	GTIN
N8016-1G	04061826692158
N8016-250MG	04061834118350