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N8523

Monoclonal Anti-NF-κB antibody produced in mouse

clone NF-12, ascites fluid

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00802846
Conjugate:
unconjugated
Clone:
NF-12, monoclonal
Application:
immunocytochemistry
microarray
western blot
Species reactivity:
human, mouse
Citations:
13
Technique(s):
immunocytochemistry: suitable
microarray: suitable
western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line
Uniprot accession no.:
P19838

Key Documents

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Product Name

Monoclonal Anti-NF-κB antibody produced in mouse, clone NF-12, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NF-12, monoclonal

mol wt

antigen 65 kDa

contains

15 mM sodium azide

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
microarray: suitable
western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line

isotype

IgG1

UniProt accession no.

P19838

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... NFKB1(4790)
mouse ... Nfkb1(18033)

Application

Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.

Immunogen

recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000245236
0000239421
0000239421
0000245236
0000109896

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Effects of simvastatin and taurine on delayed cerebral vasospasm following subarachnoid hemorrhage in rabbits
Cheng Lin
Experimental and Therapeutic Medicine, 1355-1360 (2016)
Min Hu et al.
International journal of clinical and experimental pathology, 11(4), 2072-2079 (2018-04-01)
In the establishment and continuation of a successful pregnancy, the proliferation, migration, and invasion of trophoblast cells play essential roles. Impaired trophoblast function has been reported to be involved in recurrent spontaneous abortion (RSA) but the potential mechanisms are still
Chia-Hsiung Chen et al.
The international journal of neuropsychopharmacology, 15(1), 77-90 (2011-02-19)
Elevated levels of β-site APP cleaving enzyme 1 (BACE1) were found in the brain of some sporadic Alzheimer's disease (AD) patients; however, the underlying mechanism is unknown. BACE1 cleaves β-amyloid precursor protein (APP) to generate amyloid β protein (Aβ), a
Cheng Lin et al.
Experimental and therapeutic medicine, 11(4), 1355-1360 (2016-04-14)
The aim of the current study was to observe the effects of simvastatin and taurine on delayed cerebral vasospasm (DCVS) following experimental subarachnoid hemorrhage (SAH) in rabbits. A total of 48 New Zealand white rabbits were allocated at random into
A Combination of Podophyllotoxin and Rutin Attenuates Radiation Induced Gastrointestinal Injury by Negatively Regulating NF-?B/p53 Signaling in Lethally Irradiated Mice
Bhargab Kalita
PLoS ONE, 11(12), e0168525-e0168525 (2016)
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Data Sheet - N8523

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