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N8523

Monoclonal Anti-NF-κB antibody produced in mouse

clone NF-12, ascites fluid

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00802846
Conjugate:
unconjugated
Clone:
NF-12, monoclonal
Application:
ARR, ICC, WB
Citations:
13

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NF-12, monoclonal

mol wt

antigen 65 kDa

contains

15 mM sodium azide

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line

isotype

IgG1

UniProt accession no.

P19838

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... NFKB1(4790)
mouse ... Nfkb1(18033)

General description

Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.

Immunogen

recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).

Application

Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
动植物来源生物产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000245236
0000239421
0000239421
0000245236
0000109896

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NF-kappaB family of transcription factors: central regulators of innate and adaptive immune functions
Caamano J and Hunter C A
Clinical Microbiology Reviews, 15(3), 414-429 (2002)
Deletion of nuclear factor-?B p50 upregulates fatty acid utilization and contributes to an anti-obesity and high-endurance phenotype in mice.
Minegishi Y
American Journal of Physiology. Endocrinology and Metabolism, 309(6), E523-E533 (2015)
Loss of Nfkb1 leads to early onset aging
Giovanna M. Bernal
Aging, 6(11), 931?942-931?942 (2014)
Chia-Hsiung Chen et al.
The international journal of neuropsychopharmacology, 15(1), 77-90 (2011-02-19)
Elevated levels of β-site APP cleaving enzyme 1 (BACE1) were found in the brain of some sporadic Alzheimer's disease (AD) patients; however, the underlying mechanism is unknown. BACE1 cleaves β-amyloid precursor protein (APP) to generate amyloid β protein (Aβ), a
Cortical Structure Alterations and Social Behavior Impairment in p50-Deficient Mice
Sara Anna Bonini
Cerebral Cortex, 26(6), 2832?2849-2832?2849 (2016)
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Data Sheet - N8523

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