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Merck
CN

N8786

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal

clone 414, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD02263844
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

414, monoclonal

form

buffered aqueous solution

mol wt

antigen 62-210 kDa

species reactivity

mouse, rat, yeast, Xenopus, human

concentration

~3 mg/mL

technique(s)

electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using HeLa total cell extract

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NPIPB15(440348)

General description

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.
Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.

Immunogen

rat liver nuclei extract.

Application

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunoelectron microscopy
  • immunofluorescence

Biochem/physiol Actions

Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000139092
0000110237
0000195437
0000110237
0000139092

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Isolation of the yeast nuclear pore complex.
Rout MP and Blobel G
The Journal of cell biology, 123(4), 771-783 (1993)
The nuclear pore complex ? structure and function at a glance
Greg Kabachinski
Journal of Cell Science (2015)
EGF signaling and the origin of axial polarity among the insects
Lynch JA, et al.
Current Biology, 20(11), 1042-1047 (2010)
Lentiviral Vpx induces alteration of mammalian cell nuclear envelope integrity
Singh SP, et al.
Biochemical and biophysical research communications, 511(1) (2019)
Natalia Wesolowska et al.
eLife, 9 (2020-01-29)
The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) that are stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an
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