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N9284

Nitroreductase from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli

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Synonym(s):

NTRA

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD03457727
Specific activity:
≥100 units/mL
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Segment

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100 units/mL

mol wt

monomer 24000

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Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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UniProt accession no.

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shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... nfsB(945483)

General description

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Lyophilized powder containing PBS. Does not contain BSA as excipient

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Preparation Note

Produced using animal component-free materials.

Other Notes

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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