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N9389

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

tablet

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Synonym(s):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
Beilstein:
5324661
EC Number:
224-246-5
MDL number:
MFCD00066288
PubChem Substance ID:
24897898
NACRES:
NA.83

description

5 mg substrate per tablet

Quality Level

300

form

tablet

fluorescence

λex 310 nm (pH 7.1)
λex 399 nm (Reaction Product)

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
  • enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
  • enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
  • alkaline phosphatase assay

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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J Eckl-Dorna et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 42(9), 1347-1355 (2012-08-29)
The production of allergen-specific IgE antibodies is a hallmark of IgE-mediated allergy but the contribution of blood cells to allergen-specific IgE production in allergic patients has not been studied in detail. Aim of this study was the characterization of IgE-producing
Xiaohong Wang et al.
Biochemical pharmacology, 102, 97-106 (2016-01-06)
Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the
Marianne K Schesny et al.
Biomaterials, 35(25), 7180-7187 (2014-05-31)
Chemokine-induced stem cell recruitment is a promising strategy for post myocardial infarction treatment. Injection of stromal cell-derived factor 1 (SDF1) has been shown to attract bone marrow-derived progenitor cells (BMPCs) from the blood that have the potential to differentiate into
Rasmus Iversen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17146-17151 (2014-11-19)
The multifunctional enzyme transglutaminase 2 (TG2) is the target of autoantibodies in the gluten-sensitive enteropathy celiac disease. In addition, the enzyme is responsible for deamidation of gluten peptides, which are subsequently targeted by T cells. To understand the regulation of
Masaya Yokota et al.
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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