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N9784

Sigma-Aldrich

Neutralization Solution for Blood

sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

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Synonym(s):
DNA neutralization solution, Neutralization buffer DNA extraction
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 1000 reactions

storage temp.

room temp

Related Categories

General description

Neutralization Solution for Blood is used along with lysis solution for blood (L3289) for the rapid extraction and neutralization of human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Neutralization Solution for blood has been used to neutralize extracted DNA prior to polymerase chain reaction (PCR) amplification.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Martina Veith et al.
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Alpha-1-antitrypsin deficiency (AATD) is a rare hereditary condition resulting from the mutations in the SERPINA1 (serine protease inhibitor) gene and is characterized by low circulating levels of the alpha-1 antitrypsin (AAT) protein. The traditional algorithm for laboratory testing of AATD
G Henderson et al.
Archives of disease in childhood. Fetal and neonatal edition, 94(2), F124-F128 (2007-09-05)
The inflammatory cytokine cascade is implicated in the pathogenesis of necrotising enterocolitis (NEC). Genetic association studies of cytokine polymorphisms may help to detect molecular mechanisms that are causally related to the disease process. To examine associations between the common genetic
Sabrina M Rodriguez et al.
The Journal of general virology, 90(Pt 11), 2788-2797 (2009-07-10)
Previous studies have classified the env sequences of bovine leukemia virus (BLV) provirus from different locations worldwide into between two and four genetic groupings. These different studies gave unique names to the identified groups and no study has yet integrated
Amber K Bowers et al.
Genetics, 164(4), 1345-1353 (2003-08-22)
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These markers will allow the rapid association of a candidate gene sequence
Aaron Zhong et al.
STAR protocols, 1(2) (2020-10-20)
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made

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