NA9604

GenElute^™ HP 96-Well Plasmid Miniprep Kit

Name: GenElute<SUP>™</SUP> HP 96-Well Plasmid Miniprep Kit
Brand: SIGMA

sufficient for 4 96-well plate purifications

Synonym(s):

96 well plasmid extraction kit, 96 well plasmid isolation kit, 96 well plasmid purification kit, High Throughput Purification, Plasmid Purification, high throughput miniprep kit, high throughput plasmid kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute, GenElute^™ HP Plasmid Kit

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About This Item

NACRES:

NA.52

UNSPSC Code:

41105501

Key Documents

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Product Name

GenElute^™ HP 96-Well Plasmid Miniprep Kit, sufficient for 4 96-well plate purifications

usage

sufficient for 4 96-well plate purifications

technique(s)

DNA purification: suitable

storage temp.

15-25°C

Quality Level

200

Related Categories

Nucleic Acid Purification

Plasmid DNA | Plasmid Purification Kits

General description

Click here to view the kit protocol

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.

The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

The GenElute HP 96-Well Plasmid Miniprep Kit offers a simple, rapid, cost effective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Application

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Features and Benefits

Up to 10 μg of high-copy plasmid DNA per well
Flexibility of both manual and automated formats
Optimized for production of virus titers from MISSION shRNA plasmid vectors
No phenol/chloroform extraction or alcohol precipitation required

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS02,GHS08,GHS05,GHS07

signalword

Danger

hcodes

H226,H290,H302,H314,H334,H336

pcodes

P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

77.0 °F

flash_point_c

25 °C

Regulatory Information

危险化学品

低风险生物材料

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High-Throughput Generation of In Silico Derived Synthetic Antibodies via One-step Enzymatic DNA Assembly of Fragments.

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Phage-display technology offers robust methods for isolating antibody (Ab) molecules with specificity for different target antigens. Recent advancements couple Ab selections with in silico strategies, such as predictive computational models or next-generation sequencing metadata analysis of Ab selections. These advancements

Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer.

Chunhua Wan et al.

Science advances, 7(21) (2021-06-18)

Aberrant activation of Wnt/β-catenin pathway is a key driver of colorectal cancer (CRC) growth and of great therapeutic importance. In this study, we performed comprehensive CRISPR screens to interrogate the regulatory network of Wnt/β-catenin signaling in CRC cells. We found

GenElute^™ HP 96-Well Plasmid Miniprep Kit