NA9604
GenElute™ HP 96-Well Plasmid Miniprep Kit
sufficient for 4 96-well plate purifications
Synonym(s):
96 well plasmid extraction kit, 96 well plasmid isolation kit, 96 well plasmid purification kit, High Throughput Purification, Plasmid Purification, high throughput miniprep kit, high throughput plasmid kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute, GenElute™ HP Plasmid Kit
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
usage
sufficient for 4 96-well plate purifications
Quality Level
technique(s)
DNA purification: suitable
storage temp.
15-25°C
Related Categories
General description
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.
The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.
The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.
The GenElute HP 96-Well Plasmid Miniprep Kit offers a simple, rapid, cost effective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
Application
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
Features and Benefits
- Up to 10 μg of high-copy plasmid DNA per well
- Flexibility of both manual and automated formats
- Optimized for production of virus titers from MISSION shRNA plasmid vectors
- No phenol/chloroform extraction or alcohol precipitation required
Packaging
Components included:
- Qty in kit:4 Part number: CLS3879 Component description: Elution Plate (Corning® Costar®cell culture plate)
- Qty in kit:2 Part number: E7777 Component description: Elution Solution (10 mM Tris-HCl, pH 8.5)
- Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution
- Qty in kit:1 Part number: F3555 Component description: Filter Plate
- Qty in kit:1 Part number: R1149 Component description: Resuspension Solution
- Qty in kit:1 Part number: B6686 Component description: Binding Plate
- Qty in kit:1 Part number: N1285 Component description: Neutralization Solution
- Qty in kit:1 Part number: L1912 Component description: Lysis Solution
- Qty in kit:1 Part number: W4639 Component description: Wash Solution 2
- Qty in kit:1 Part number: R6148 Component description: RNase A solution
- Qty in kit:1 Part number: W0263 Component description: Wash Solution 1
- Qty in kit:1 Part number: A2350 Component description: AlumaSeal® II film
- Qty in kit:1 Part number: B4683 Component description: Binding Solution
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
77.0 °F
Flash Point(C)
25 °C
Regulatory Information
危险化学品
低风险生物材料
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