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O1391

Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

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Synonym(s):
Solvent Red 27, Sudan Red 5B
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
1320-06-5
Molecular Weight:
408.49
EC Number:
200-661-7
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

0.5% in isopropanol

color

dark red

εmax

0.20-0.50 at 510-517 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

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General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319 - H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup

Regulatory Information

危险化学品

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Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice.
Zhang W, et al.
Alcoholism, Clinical and Experimental Research, 40(5), 988-999 (2016)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
Wu W, et al.
Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)
Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Li Q, et al.
Breast Cancer Research and Treatment, 141(2), 317-324 (2013)
LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)
Pablo Sanchez Bosch et al.
Developmental cell, 51(6), 787-803 (2019-11-19)
The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and
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