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O4878

Oxaloacetate Decarboxylase from Pseudomonas sp.

lyophilized powder, ≥100 units/mg solid

Synonym(s):

OAD

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About This Item

CAS Number:
9024-98-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
4.1.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00131775
Specific activity:
≥100 units/mg solid
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Quality Segment

200

form

lyophilized powder

specific activity

≥100 units/mg solid

storage temp.

−20°C

General description

Oxaloacetate decarboxylase or OAD functions as a Na pump in anaerobic bacteria. It is a membrane protein consisting of three subunits, α, β and γ with the α subunit containing the carboxylase activity.

Application

Oxaloacetate Decarboxylase from Pseudomonas sp. has been used in the digestion of the low molecular weight (LMW) human milk fraction (5kF fraction) and as a positive control for deciphering C. thermocellum oxaloacetate decarboxylase activity.
Oxaloacetate decarboxylase has been used in a study to assess turnover and accessibility of a reentrant loop of the Na(+)-glutamate transporter GltS. It has also been used in a study to investigate fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources.

Biochem/physiol Actions

Oxaloacetate Decarboxylase catalyzes the decarboxylation of oxaloacetate and requires manganese and magnesium for its activity. It is associated with a wide vareity of Gram-negative bacteria.

Other Notes

One unit will convert 1.0 μmole of oxalacetate to pyruvate and CO2 per min at pH 8.0 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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