O9515
Prolyl oligopeptidase
recombinant, expressed in E. coli
Synonym(s):
Prolyl endopeptidase
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥10 unit/μg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
solution
specific activity
≥10 unit/μg protein
mol wt
81.6 kDa
shipped in
dry ice
storage temp.
−70°C
Quality Level
Related Categories
General description
Prolyl oligopeptidase (PO) is mapped to human chromosome 6q22. It comprises of a N-terminal β-propeller domain and C-terminal α/β hydrolase catalytic domain. PO is highly expressed in the neuronal cytoplasm and its expression increases with age.
Prolyl oligopeptidase is a cytosolic serine peptidase which cleaves peptide bonds at the C′ terminal side of prolines. It is only capable of processing peptides containing no more than 30 amino acids due to the unique β-propeller region that regulates access to the active site.
Application
Prolyl oligopeptidase has been used in a study to assess the mechanism of lithium ion action. It has also been used in a study to investigate its distribution in human tissue and body fluids.
Prolyl oligopeptidase has been used in the prolyl oligopeptidase inhibitory activity assay in lyophilized protein hydrolysate samples and Schistosoma mansoni samples.
Biochem/physiol Actions
Prolyl oligopeptidase (PO) regulates physiological processes and is crucial for the generation of active hormone and peptide fragments from their precursors. It shows elevated levels in psychiatric disorders and Alzheimer′s patients. Altered levels of PO is observed in patients with bipolar disorder. High expression of PO may promote metastasis of malignant ovarian and colorectal tumors.
Physical form
Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT and variable amounts of imidazole.
Preparation Note
N-terminal GST-tagged 81.6 kDa full-length protein
Other Notes
One unit will hydrolyze 1.0 picomole of Ala-Pro-aminomethylcoumarin per minute at pH 7.4 at 25 °C.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Mechanism of Action of Prolyl Oligopeptidase (PREP) in Degenerative Brain Diseases: Has Peptidase Activity Only a Modulatory Role on the Interactions of PREP with Proteins?
Mannisto PT, et al.
Frontiers in Aging Neuroscience, 9(3), 27-27 (2017)
Structure-function properties of prolyl oligopeptidase family enzymes
Rea D and Fulop V
Cell Biochemistry and Biophysics, 44(3), 349-365 (2006)
R S Williams et al.
The EMBO journal, 18(10), 2734-2745 (1999-05-18)
The therapeutic properties of lithium ions (Li+) are well known; however, the mechanism of their action remains unclear. To investigate this problem, we have isolated Li+-resistant mutants from Dictyostelium. Here, we describe the analysis of one of these mutants. This
Distribution of prolyl oligopeptidase in human peripheral tissues and in ovarian and colorectal tumors
Myohanen TT, et al.
The Journal of Histochemistry and Cytochemistry, 60(9), 706-715 (2012)
Prolyl oligopeptidase and bipolar disorder
Williams, Robin SB
Clinical neuroscience research, 4(3-4), 233-242 (2004)
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