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Merck
CN

O9755

Oil Red O

BioReagent, suitable for electrophoresis

Synonym(s):

1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27

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About This Item

Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
UNSPSC Code:
41116100
NACRES:
NA.32
PubChem Substance ID:
EC Number:
215-295-3
MDL number:
Colour Index Number:
26125
eCl@ss:
32150313
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product line

BioReagent

Quality Segment

form

powder

technique(s)

electrophoresis: suitable

mp

120 °C (dec.) (lit.)

λmax

359 nm, 518 nm (2nd)

suitability

suitable for electrophoresis

SMILES string

Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3

InChI key

NPGIHFRTRXVWOY-UHFFFAOYSA-N

Application

Oil Red O stain is suitable as a lipid/lipoprotein stain on cellulose acetate. A 0.2% stock solution is prepared in methanol. The stock solution (35 ml) is diluted with 10 mL of 1 M NaOH immediately before staining. The dye begins precipitating during staining. After staining (1 hr), the membrane is destained in methanol:glycerol (1:3). Often cited for use with agarose


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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