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P0001

Phosphatase Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Synonym(s):

Phosphatase inhibitor cocktail

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
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Product Name

ReadyShield® Phosphatase Inhibitor Cocktail, For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Quality Level

200

form

ready-to-use solution

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

solubility

water: soluble

storage temp.

−20°C

Related Categories

General description

This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000506114
0000506116
0000506116
0000506114
0000430792

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Global Trade Item Number

SKUGTIN
P0001-1ML04061842555161
P0001-5ML04061842555178

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