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Merck
CN

P0089

Anti-Prox1 antibody , Mouse monoclonal

clone 4G10, purified from hybridoma cell culture

Synonym(s):

Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD09039204

Key Documents

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Product Name

Anti-Prox1 antibody , Mouse monoclonal, clone 4G10, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4G10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~84 kDa

species reactivity

chicken, rat, lizard, human

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells
western blot: suitable

isotype

IgG2b

UniProt accession no.

Q92786

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... PROX1(5629)
rat ... Prox1(305066)

Application

Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.

Biochem/physiol Actions

Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.

Immunogen

recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000290406
0000290406
0000182483
0000182463
22180522

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Loss of Function of the Candidate Tumor Suppressor prox1 by RNA Mutation in Human Cancer Cells
Takahashi M, et al.
Neoplasia, 8(12), 1003-1010 (2006)
Xiaoren Chen et al.
Hybridoma (2005), 25(1), 27-33 (2006-02-16)
Prox1 is a divergent homeodomain protein important for the development of the lens, retina, liver, pancreas, and lymphatic vasculature. Prox1 expression is highly upregulated in transformed hepatocytes and has been used as a marker to distinguish lymphatic from blood vasculature.
Molecular mechanisms of lymphatic vascular development
Makinen T, et al.
Cellular and Molecular Life Sciences, 64(15), 1915-1929 (2007)
Epigenetic silencing of the candidate tumor suppressor gene PROX1 in sporadic breast cancer
Versmold B, et al.
International Journal of Cancer. Journal International Du Cancer, 121(3), 547-554 (2007)
Guadalupe Álvarez-Hernán et al.
Cells, 12(7) (2023-04-14)
The quail (Coturnix coturnix, Linnaeus 1758), a notable model used in developmental biology, is a precocial bird species in which the processes of retinal cell differentiation and retinal histogenesis have been poorly studied. The purpose of the present research is
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Data Sheet - P0089

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