P0157
Phosphatase, Acid from potato
ammonium sulfate suspension, ≥200 units/mg protein (biuret)
Synonym(s):
(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
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About This Item
CAS Number:
EC Number:
232-630-9
UNSPSC Code:
12352204
MDL number:
eCl@ss:
42010102
form
ammonium sulfate suspension
specific activity
≥200 units/mg protein (biuret)
mol wt
111 kDa
foreign activity
Apyrase (ATPase) ≤5% of base activity
storage temp.
2-8°C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
General description
Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.
Application
Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.
Physical form
Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.5
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Related Content
Y Mikuni-Takagaki et al.
The Biochemical journal, 268(3), 585-591 (1990-06-15)
In order to understand the mechanism of the post-translational processing of bone phosphoproteins in embryonic bone, periosteal bone strips isolated from 12-day-embryonic-chick tibiae were cultured and the bone proteins labelled with Na2H32PO4. Of the total radiolabelled proteins recovered from the
Insight into bio-physiological functions of acid phosphatase from potatoes (Solanum tuberosum): A bioanalytical approach.
Rane, S.
Indian Journal of Chemistry, 48a, 15-24 (2009)
The kinetics and mechanism of the hydrolysis of phosphoric acid esters by potato acid phosphatase.
E F ALVAREZ
Biochimica et biophysica acta, 59, 663-672 (1962-06-04)