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Merck
CN

P0498

Anti-PRL-3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PTP4A3, Anti-Phosphatase of Regenerating Liver

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD07370459
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC (p), IP, WB CL
Citations:
9

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250-1:500 using protease digested tissue sections from human heart by biotin/ExtrAvidin®-peroxidase staining, immunoprecipitation (IP): 0.5-1 μL using preparation containing recombinant human PRL-3, western blot (chemiluminescent): 1:500-1:1,000 using whole extract of human HepG2 cells

UniProt accession no.

O75365

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... PTP4A3(11156)
mouse ... Ptp4a3(19245)

General description

Phosphatase of regenerating liver (PRL-3), also known as protein tyrosine phosphatase type IVA, member 3 (PTP4A3) is a dual-specificity phosphatase. It belongs to the phosphatases of regenerating liver family. The PTP4A3 gene is localized on human chromosome 8q24.3.

Immunogen

Synthetic peptide corresponding to amino acid residues of human PRL-3 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-PRL-3 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Anti-PRL-3 recognizes human phosphatase of regenerating live (PRL)-3 and does not cross-react with human PRL-1 and PRL-2.
Phosphatase of regenerating liver (PRL-3) promotes epithelial-mesenchymal transition. It also plays an essential role in cancer cell migration, invasion and metastasis. It dephosphorylates serine, threonine and tyrosine residues. The protein is overexpressed in various cancers. PTP4A3 associates with transcription factors and regulates gene transcription. In addition, it also regulates breast cancer cell proliferation and invasion. Thus, this protein acts as a potent therapeutic target for breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
128M4813V
21180110
074M4766V
073M4766V
104K4792

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Expression of PRL-3 regulates proliferation and invasion of breast cancer cells in vitro.
Radke I, et al.
Archives of Gynecology and Obstetrics (2017)
Yi Cao et al.
Molecular therapy. Nucleic acids, 19, 853-864 (2020-01-27)
Gastric cancer (GC) is among the most frequently occurring malignancies worldwide. In recent years, long noncoding RNAs (lncRNAs) have been widely studied because of their ability to regulate the cellular processes involved with tumorigenesis. The present study aims to investigate
High PTP4A3 Phosphatase Expression Correlates with Metastatic Risk in Uveal Melanoma Patients
Laurent C, et al.
Cancer Research, 71(3), 666-674 (2011)
Expression of phosphatase of regenerating liver (PRL)-3, is independently associated with biochemical failure, clinical failure and death in prostate cancer.
Andersen S, et al.
PLoS ONE, 12(11), e0189000-e0189000 (2017)
Src-Mediated Phosphorylation of the Tyrosine Phosphatase PRL-3 Is Required for PRL-3 Promotion of Rho Activation, Motility and Invasion
Fiordalisi JJ, et al.
PLoS ONE, 8(5), e64309-e64309 (2013)
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