P0609
Pepsin−Agarose from porcine gastric mucosa
lyophilized powder, ≥30 units/mg dry solid
Synonym(s):
Pepsin A
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About This Item
Specific activity:
≥30 units/mg dry solid
Biological source:
Porcine gastric mucosa
biological source
Porcine gastric mucosa
Quality Segment
form
lyophilized powder
specific activity
≥30 units/mg dry solid
mol wt
35 kDa
matrix
4% cross-linked beaded agarose
UniProt accession no.
storage temp.
−20°C
Gene Information
pig ... LOC396892(396892)
Application
Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.
Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.
Used to produce F(ab′)2 fragments of antibodies.1
Biochem/physiol Actions
Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.
Physical form
Lyophilized powder stabilized with lactose
Preparation Note
One gram solid swells to 5-10ml packed gel.
Other Notes
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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