P1093
Monoclonal Anti-Paxillin antibody produced in mouse
clone PXC-10, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
PXC-10, monoclonal
mol wt
antigen 68-40 kDa
contains
15 mM sodium azide
species reactivity
bovine, rat, human, chicken, hamster
technique(s)
immunocytochemistry: suitable
microarray: suitable
western blot: 1:500 using a cultured human cell line extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PXN(5829)
rat ... Pxn(360820)
General description
Monoclonal Anti-Paxillin (mouse IgG1 isotype) is derived from the PXC-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Paxillin (68 kDa), is a cytoskeletal component. It is found in the ends of actin stress fibers of the focal adhesions, but not in adherens junctions of the cells. The paxillin molecule has a single binding site for vinculin, and at least two binding sites for focal adhesion kinase (FAK), that are separated by an intervening sequence of 100 amino acids.
Immunogen
C-terminal part of recombinant chicken paxillin (amino acids 305-559)
Application
Monoclonal Anti-Paxillin antibody produced in mouse has been used in immunoblotting and immunocytochemistry.
Biochem/physiol Actions
Paxillin interacts with several proteins including members of the src family of tyrosine kinases, the transforming protein v-crk, the cytoskeletal protein vinculin, and the tyrosine kinase, focal adhesion kinase (FAK). This interaction has suggested a function for paxillin as a molecular adaptor, responsible for the recruitment of structural and signaling molecules to focal adhesions.
Recognizes an epitope located in the LIM1 domain of the paxillin molecule.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Paxillin: a new vinculin-binding protein present in focal adhesions.
Turner CE, et al.
The Journal of cell biology, 111(3), 1059-1068 (1990)
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