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P1167

Pituitary Extract bovine

≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

Synonym(s):

BPE, PEX

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
Biological source:
bovine
Form:
lyophilized powder
Impurities:
≤50 EU/mg
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Product Name

Pituitary Extract bovine, lyophilized powder, suitable for cell culture

biological source

bovine

Quality Level

form

lyophilized powder

potency

15 - 200 μg/mL EC50

quality

endotoxin tested

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mg

color

brown

solubility

0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange

storage temp.

2-8°C

General description

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.

Application

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.

Biochem/physiol Actions

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.

Physical form

Lyophilized from sodium phosphate buffer


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Related Content

Instructions


Yung-Chih Kuo et al.
Biotechnology progress, 21(6), 1708-1715 (2005-12-03)
In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with
Simon W Hayward et al.
Methods in molecular biology (Clifton, N.J.), 218, 17-33 (2003-03-06)
The ability to rescue viable prostate precursor tissue from Rb-/-fetal mice has allowed for the generation of Rb-/-prostate tissue and Rb-/-prostate epithelial cell lines. Herein, we provide a protocol for the rescue of urogenital precursor tissue from mouse embryos harboring
J C Willey et al.
Journal of cellular physiology, 124(2), 207-212 (1985-08-01)
The mechanisms of action of extracellular mitogens for normal human bronchial epithelial cells (NHBE) were investigated by observing their effects on selected biochemical pathways when the cells were incubated in serum-free media. We find that (a) epidermal growth factor (EGF)



Global Trade Item Number

SKUGTIN
P1167-5MG04061834354185