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P1473

Sigma-Aldrich

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

Synonym(s):

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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1 EA
CN¥2,782.63

CN¥2,782.63

Estimated to ship onOctober 09, 2025Details

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1 EA
CN¥2,782.63

About This Item

UNSPSC Code:
41141827
NACRES:
NA.25

CN¥2,782.63

Estimated to ship onOctober 09, 2025Details

grade

Molecular Biology

Quality Level

200

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Application

PCR 100 bp Low Ladder has been used:
  • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis[1]
  • to visualize DNA from enzyme digested PCR mixture[2]
  • to estimate the molecular weight of the PCR product fragments[3]

Components

Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000420136
    0000420136
    0000364605
    0000364605
    SLCL6918

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