P1473

PCR 100 bp Low Ladder

Name: PCR 100 bp Low Ladder
Brand: SIGMA

for electrophoresis of PCR fragments

Synonym(s):

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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About This Item

UNSPSC Code:

41141827

NACRES:

NA.25

Key Documents

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biological source

synthetic

Quality Level

200

grade

Molecular Biology

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Application

PCR 100 bp Low Ladder has been used:

in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
to visualize DNA from enzyme digested PCR mixture
to estimate the molecular weight of the PCR product fragments

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

This item has

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Certificates of Analysis (COA)

Lot/Batch Number

0000420136

0000364605

SLCL6918

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Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

Select a Size

About This Item

Key Documents

Properties

biological source

Quality Level

grade

form

usage

suitability

storage temp.

Description

General description

Application

Other Notes

Safety Information

Storage Class Code

WGK

Flash Point(F)

Flash Point(C)

Personal Protective Equipment

Regulatory Information

Documentation

Certificates of Analysis (COA)

Already Own This Product?

Peer Reviewed Papers

Protocols and Articles

Articles

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