P1588
Placenta Growth Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
PGF, PlGF
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
29 kDa
Impurities:
<1.0 EU/μg
Product Name
Placenta Growth Factor human, PlGF, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
powder
quality
endotoxin tested
mol wt
29 kDa
packaging
pkg of 10 and 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1.0 EU/μg
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
Placenta growth factor (PlGF) is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.
Preparation Note
Recombinant human PlGF is lyophilized from a 0.2 micron filtered solution of 30% CH3CN and 0.1% TFA (pH 7.4) containing 50 microg of bovine serum albumin per 1 microg of cytokine.
Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.
Analysis Note
The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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